Enzyme Inhibition - Oct 9th

Outcomes

  • Utlilize various graphical representations of the M-M rate equation to distinguish between mechanisms for multiple substrate reactions
  • Utlilize various graphical representations of the M-M rate equation to determine inhibition type
  • Determine Km, Vmax, and the inhition constant Ki using plots of the M-M equation
  • Discuss the relationship between substrate binding site and active site and the values of Vmax and Km

Reading (to be completed before class!)

Lehninger Chp 6, Sect. 6.3 (Inhibition covered from 206-212)

  • Know and understand all terms in bold
  • Match Inhibition type to representations in Fig 6-17
  • Understand terms in Equations 6-28, -29, -30)
  • Know tests for Dtermining Inhibition Mechanisms Box 6-2

On Reactions with Two or More Substrates

  • Know terms in bold
  • Know how to interpret
    • Fig 6-13
    • Fig 6-14

Pre-Assignment

Pre-Assignment activities on Worksheet forInhibition (please email photos of your answers) and be prepared to show your reading log in class.

In Class Activities

  • Worksheet on Inhibition
  • Slideshow on using LB plots to distinguish types of Inhibition and and types Multiple substrate reactions

Problems

  • All Post-Activity Exercises on Enzyme Inhibition Worksheet
  • Lehninger Chp 6: 6, 7, 8, 9, 10, 11, 13, 14 (use Excel), 21, 24