Folding Thermodynamics and Kinetics - April 6th

Reading (to be completed before class!)

  • Lehninger p143-146
    • (this will provide a VERY brief overview of the topics (see goals)
  • Whitford, Chp 11, pp - 395-403
    • Fills in details on thermodynamics of folding and unfolding
  • Berg Ch6 3.6.2
    • I added this for the nice discussion on Cooperativity in Protein Folding

Pre-Assignment
Create an outline of the reading.
Within the outline, highlight two questions to share in class discussion.

Goals

In the next three sections we will look at three broad topics

  • Thermodynamics and equilibrium unfolding and stability of proteins
  • Folding Pathways and Kinetics of Folding
  • Determinants of Protein Folding
  • Techniques to measure folding

In Class Activities

Discussion

Discussion Slides (movie)

Past Class Outline

 Note: I supplemented my discussion of the reading from three sources (focus on the highlighted parts)
  •  Fersht. The sixth Datta Lecture. Protein folding and stability: the pathway of folding of barnase. FEBS Lett (1993) vol. 325 (1-2) pp. 5-16
  •  Gutin et al . A protein engineering analysis of the transition state for protein folding: simulation in the lattice model. Fold Des (1998) vol. 3 (3) pp. 183-94

  • Apetri and Surewicz. Kinetic intermediate in the folding of human prion protein. J Biol Chem (2002) vol. 277 (47) pp. 44589-92
    • We used the first pages of ths paper in class to expand on the discussion of the Chevron plots discussed in Whitford