Denaturation Paper / Unfolding Kinetics- April 11th

Catch-up day

We will conitinue our discussion of

Folding of the SARS coronavirus spike glycoprotein immunological fragment (SARS_S1b): thermodynamic and kinetic investigation correlating with three-dimensional structural modeling. Yu et al. Biochemistry (2005) vol. 44 (5) pp. 1453-63 (http://pubs.acs.org/doi/abs/10.1021/bi0482396)

We will also go back to our discussion of unfolding kinetics

Whitford, Chp 11, pp - 404-415

Nice dscussion of the kinetics of protein folding and Leninthal's Paradox

Note: I supplemented my discussion of the reading from three sources (focus on the highlighted parts)

  •  Fersht. The sixth Datta Lecture. Protein folding and stability: the pathway of folding of barnase. FEBS Lett (1993) vol. 325 (1-2) pp. 5-16
  •  Gutin et al . A protein engineering analysis of the transition state for protein folding: simulation in the lattice model. Fold Des (1998) vol. 3 (3) pp. 183-94
  • Apetri and Surewicz. Kinetic intermediate in the folding of human prion protein. J Biol Chem (2002) vol. 277 (47) pp. 44589-92
    • We used the first pages of ths paper in class to expand on the discussion of the Chevron plots discussed in Whitford

Problems

See Past assignment pages for problems.