Denaturation Paper - April 9th

Reading (to be completed before class!)

Review Reading

Folding of the SARS coronavirus spike glycoprotein immunological fragment (SARS_S1b): thermodynamic and kinetic investigation correlating with three-dimensional structural modeling.

Yu et al. Biochemistry (2005) vol. 44 (5) pp. 1453-63

or

http://pubs.acs.org/doi/abs/10.1021/bi0482396

Problems

 

Questions for class discussion

In Class Activities

Discuss paper -

Each group should present the figures from within their section and lead discussion of the meaning and significance of the results. You may need to refer to the experimental section or past class material and lectures on fluorsecnce CD, denaturation, etc.

You have to read all sections. But you will lead the discussion of your sections. I will have the figures displayed on the overhead for you to refer to.

Each team will have 3 minutes/person to present their section(s).


INTRODUCTION

  • (including figure 1) led by ...

RESULTS and DISCUSSION

  • Intrinsic Tryptophan Fluorescence (all of figure 2 and Table 1) - led by 
  • Temperature-Dependent CD Spectra (all of figure 3) led b
  • Unfolding under Equilibrium Conditions (including figure 4 and Table 1) - led by
  • Unfolding and Refolding Kinetics - (figure 5 and 6) led by
  • Structural Predictions - (including Figure 7) led by