Methods II - Fluorescence and CD

Reading (to be completed before class!)

These will also be helpful

Pre-Assignment (Due in Class) Key

Why use Circular Dichroism ?

  • determination of the secondary structure
  • tertiary structure
  • folding kinetics
  • determine whether an expressed, purified protein is folded
  • if a mutation affects its conformation or stability.
  • protein-protein interactions
  • ligand binding
  • CD measurementent
    Basic experiment.
  • Protein secondary structure determination

In Class Activities

  • Group discussion on UV-vis and fluorescence and CD applications
  • Class notes (from a previous class)

 

Problems

Fluorescence and CD problems