Thermodynamics - Feb 23

Reading (to be completed before class!)

Helpful Review
Voet 4th ed
Chp 3: all
Chp 8, Sect 4

In Class Activities

Problems

Voet Chp 3: 3, 9, 13
Voet Chp 8: 13, 14

Additional Problems: See worksheet

Interpret the table below to answer the following questions (adapted from Fiore et al. Biochem. 2009, 48, 2550): Thermodynamic parameters for folding of structured RNAs

  • Is the folding of these RNA molecules driven by the same forces as the peptides discussed in class? Justify your answer.
  • Calculate the ΔGo for each RNA (assume 298K). Is folding spontaneous?
     RNA  ΔHo (kcal/mol)  ΔSo (cal/mol*K)
    P4-P6 domain of Group I intron  -28 ± 3  -91 ± 8
       -4.1 ± 2.3  -9.6 ± 5.7