Reading (to be completed before class!)
Prepare to lead discussion
In Class Activities
- Class produced Google Doc Summary of Group discussion of the papers
- The first section in article by Dobson reviews protein folding kinetics. Based on the reading put together a summary of the protein folding process.
- According to Dobson what parameter of the protein structure has the most significant correlation with folding rate?
- What is the "modular mechanism" of folding?
- From Taylor et al, what are the two strategies to prevent and deal with protein misfolding?
- List two enzymes involved in increasing the rate of protein folding. What reactions do they catalyze?
- Based on both papers properties do all the misfolded proteins associated with these diseases have in common? How do these the misfolded protein differ from normal, globular proteins both in structure and formation? Related to this is the fact that the ability to form fibrils is common to all proteins.
- The Dobson paper suggests that familial forms of disorders like ALS and Alzheimer's, proteins have an increased propensity to misfold. Discuss some possibilities and, when possible, link them to specific diseases.
Summary of Class Answers to the questions, from 2014