Week 9

April 8th-10th - Denaturation Paper

Reading (to be completed before class!)

Folding of the SARS coronavirus spike glycoprotein immunological fragment (SARS_S1b): thermodynamic and kinetic investigation correlating with three-dimensional structural modeling.

Yu et al. Biochemistry (2005) vol. 44 (5) pp. 1453-63




Questions taken from class discussion


In Class Activities

Discuss paper -

Each group should present the figures from within their section and lead discussion of the meaning and significance of the results. You may need to refer to the experimental section or past class material and lectures on fluorsecnce CD, denaturation, etc.

You have to read all sections. But you will lead the discussion of your sections. I will have the figures displayed on the overhead for you to refer to.

Each team will have 6-8 minutes to discuss their section(s) and the relevance of the findings to the conclusions.


  • (including figure 1) led by Sigman


  • Intrinsic Tryptophan Fluorescence (all of figure 2 and Table 1) - led by Trevor, Tessa, Shane
  • Temperature-Dependent CD Spectra (all of figure 3) led by Kayla, Amanda
  • Unfolding under Equilibrium Conditions (including figure 4 and Table 1) - led by Hans, Alina
  • Unfolding and Refolding Kinetics - (figure 5 and 6) led by Julian, Erika
  • Structural Predictions - (including Figure 7) led by Hamida, Sasha